VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
Popular shRNAs
Name | Target Sequence | Target Gene | Target Position | References | Sequence |
---|---|---|---|---|---|
Scramble[shRNA#1] | CCTAAGGTTAAGTCGCCCTCG | None in human and mouse | Cancer Res. 66:9270 (2006) | View | |
Scramble[shRNA#2] | CAACAAGATGAAGAGCACCAA | TurboGFP; none in human and mouse | Oncogene. 30:1551 (2011); Science. 343:309 (2014) | View | |
EGFP[shRNA#1] | CGACGTAAACGGCCACAAGTT | EGFP | 63-83 | The RNAi Consortium ID: TRCN0000072193 | View |
EGFP[shRNA#2] | ACGTCTATATCATGGCCGACA | EGFP | 449-469 | The RNAi Consortium ID: TRCN0000231756 | View |
LacZ[shRNA#1] | GTCGGCTTACGGCGGTGATTT | LacZ | 1734-1754 | The RNAi Consortium ID: TRCN0000072242 | View |
LacZ[shRNA#2] | GTTCCGTCATAGCGATAACGA | LacZ | 1908-1928 | The RNAi Consortium ID: TRCN0000072238 | View |
Luciferase[shRNA#1] | ATGTTTACTACACTCGGATAT | Firefly luciferase | 745-765 | The RNAi Consortium ID: TRCN0000231737 | View |
Luciferase[shRNA#2] | TGTCCGGTTATGTAAACAATC | Firefly luciferase | 1193-1213 | The RNAi Consortium ID: TRCN0000072258 | View |
Scramble[miR30-shRNA#1] | ACCTAAGGTTAAGTCGCCCTCG | None in human and mouse | Designed by VectorBuilder | View | |
Scramble[miR30-shRNA#2] | CCAACAAGATGAAGAGCACCAA | TurboGFP; none in human and mouse | Oncogene. 30:1551 (2011); Science. 343:309 (2014) | View | |
EGFP[miR30-shRNA#1] | GCGACGTAAACGGCCACAAGTT | EGFP | 62-83 | Designed by VectorBuilder | View |
EGFP[miR30-shRNA#2] | AACGTCTATATCATGGCCGACA | EGFP | 448-469 | Designed by VectorBuilder | View |
EGFP[miR30-shRNA#3] | AAGAACGGCATCAAGGTGAACT | EGFP | 475-496 | Designed by VectorBuilder | View |
EGFP[miR30-shRNA#4] | CACATGAAGCAGCACGACTTCT | EGFP | 232-253 | Designed by VectorBuilder | View |