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New Publication from VectorBuilder

MiniVec: A miniaturized plasmid backbone supporting antibiotic-free and additive-free fermentation with enhanced yield and functionality
Silk J. Shi, et al. bioRxiv. 2025 (DOI: https://doi.org/10.1101/2025.10.02.679775)

요약: MiniVec은 초소형 백본 구조를 가진 새로운 plasmid 시스템으로, 기존 plasmid에 비해 탁월한 효능, 안전성 및 제조가능성을 제공합니다. 특히, MiniVec은 일시적 형질감염, 바이러스 패키징, transposon 매개 및 CRISPR 매개 유전체 통합, 네이키드 DNA 백신 접종 등 다양한 in vitro 및 in vivo 응용 분야에서 plasmid 수율을 높이고 기능성을 향상시켰으며, 면역원성이나 독성은 나타나지 않았습니다. 따라서 MiniVec 시스템은 차세대 plasmid 플랫폼을 대표하며, 제조 비용을 절감하는 동시에 유전자 치료 응용 분야의 효능과 안전성을 향상시킵니다.

View our MiniVec™ offerings >>
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유전자전달 기술분야의 글로벌 리더로 우리의 이러한 혁신과 확장성은 종종 뉴스 기사를 생성합니다. VectorBuilder 커뮤니티에서 최신 업데이트를 확인하세요.

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VectorBuilder 고객의 소리
Alexander Atkin

Alexander Atkin

University of Cambridge, UK
I was really happy with VectorBuilder's services for my PhD project, I had approached other companies to synthesise DNA containing my gene of interest, but none of them could manage thanks to some repetitive elements, even with codon optimising. Not only were VectorBuilder able to clone it perfectly but no codon optimising was required! I was also able to design and purchase lentiviruses for expressing said gene very easily and with a pretty quick turnaround of just a few weeks total, thanks to the efforts of my local VectorBuilder representative. I would highly recommend VectorBuilder for any lentivirus/gene synthesis projects and will be using them in the future if required too.
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Dr. Pedro Fonseca

Dr. Pedro Fonseca

Karolinska Institutet, Sweden
We have been using VectorBuilder to design and produce different kinds of lentiviral and adenoviral constructs to be used on cancer research over the years. I particularly appreciate your customer support where you can get expert help with designing custom vectors tailored to your research questions. I also like the modular aspect where we can choose to order DNA, bacterial stocks and/or ready-made viral titers; coupled with the nice user interface and vector maps in your website, it makes it so much easier not only to keep track of all the constructs, but to also share them with other researchers that want to use and order those same constructs. Thank you for your services, you have helped our research projects immensely!
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James Cox

James Cox

Wolfson Inst for Biomedical Research, UK
For several years we have used VectorBuilder for generating expression constructs and custom AAVs. Designing constructs is easy – either use the website or send a request of what you want by email - detailed construct designs are usually in your inbox within 24 hrs. Prices, turnaround times and quality of product mean that ordering from VectorBuilder is just the obvious decision to make when planning experiments. I recommend their services to all my friends.
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Dr. Bogang Wu

Dr. Bogang Wu

Harvard Medical School, USA
VectorBuilder has been instrumental in supporting my research by providing timely and reliable vector cloning and virus packaging services. Joaquin is always responsive to my requests and vector design needs, efficiently addressing them with care and attention to detail. His dedication to the success of my projects has contributed to several publications. I highly recommend their services.
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Dr. Leesa Sampson

Dr. Leesa Sampson

Vanderbilt Genome Editing Resource, USA
VectorBuilder’s plasmid and virus preparation services are reliable, competitively priced, and exceptional at handling challenging constructs. Their customer service is top-notch, making them an essential part of our workflow.
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Alexander Goginashvili

Alexander Goginashvili

University of California San Diego, USA
We have successfully generated several vectors with VectorBuilder and very much hope there will be many more to come. Their website is very easy to navigate. Timing is really appealing and the prices are convenient. Special thanks to Shelley Wu, the service rep in San Diego. She is extremely helpful and approachable. Overall, VectorBuilder is a huge help!
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Julius Bogomolovas

Julius Bogomolovas

UCSD School of Medicine, USA
The straightforward, intuitive interface allowed me to design my construct, by a simply copying-and-pasting sequence of my gene and choosing the right promoter and reporter from an impressive scroll down menu. The vector and recombinant viruses were made on time at a very competitive price with no extra cost for aliquoting into working aliquots. I highly recommend VectorBuilder for a complete recombinant virus production pipeline.
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Evgenia Salta

Evgenia Salta

Katholieke Universiteit Leuven/VIB, Belgium
VectorBuilder has been the partner every researcher would wish for during those dark moments of paper revision. They offer the complete package: a wide range of viral vector design options, an extremely user-friendly and intuitive website, direct communication, personal and knowledgeable step-by-step guidance throughout the designing and ordering process, accountability, amazing discounts, unique customer support, fast shipping, and guaranteed virus titers and efficiency. So here’s my big thumbs up for a great product and service provider who has proven indispensable in addressing my research questions!
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Frank Borriello

Frank Borriello

Alloplex Biotherapeutics, USA
VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.
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Dr. Cristina Martinez Gonzalez

Dr. Cristina Martinez Gonzalez

University of Edinburgh, United Kingdom
VectorBuilder's AAV serotype panel helped us to choose the best expressing serotype for our brain area and animal model of interest. By piloting injections using a selection of serotypes provided in the panel, we obtained invaluable pilot data before ordering a large scale virus production. This allowed us to save a considerable amount of time and resources. The technical support was great, they answer our questions promptly provided great advice. VectorBuilder technical support team further help us designing and cloning our AAV viral vector. Our preliminary data allowed to map retrogradely labelled neurons in the amygdala using light-sheet microscopy. We would not hesitate to use VectorBuilder for our next projects.
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Constantine Tzouanas

Constantine Tzouanas

Massachusetts Institute of Technology, USA
VectorBuilder was fantastically helpful for our AAV experiments - wonderful for talking through our goals and proposed order, and very responsive in keeping us up-to-date on our order's progress. The AAV worked very well, and working with VectorBuilder (and especially Maria Pascual) was a very positive experience!
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Xueqing Xia

Xueqing Xia

UT MD Anderson Cancer Center, USA
We are testing different vectors for T cell therapy, and VectorBuilder provides a one stop solution for design, construct and manufacturing numerus lentiviral vectors for us. One of our vector was GMP manufactured by VectorBuilder and move to FDA approved clinical trial. We had great experience working with VectorBuilder, from communication to product QC, high level of service. 
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Dr. Garron Dodd

Dr. Garron Dodd

The University of Melbourne, Australia
VectorBuilder is truly exceptional, offering best-in-class services for viral vector generation. Their contributions are so integral to our research that I consider them an invaluable extension of our lab.
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Dr. Shaikh Nisar Ali

Dr. Shaikh Nisar Ali

Long Island University, USA
We utilized two lentiviral vectors, lenti-CYP and lenti-Ctr, from VectorBuilder Inc. in our study on Lgr5+ intestinal stem cells. The lenti-CYP vector, encoding the hCYP27B1 gene, enabled de novo synthesis of locally high 1,25(OH)2D concentrations, proving crucial for our investigation. The high-quality design, including promoter specificity and integrated resistance genes, ensured reliable gene expression and successful transduction. The control vector, lenti-Ctr, further allowed precise experimental comparisons. VectorBuilder's products significantly contributed to the success of our research, and I highly recommend their custom vector services for advanced molecular biology applications.
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Dr. George-Lucian Moldovan

Dr. George-Lucian Moldovan

The Pennsylvania State University, USA
We were working on nascent DNA processing at stalled replication forks in BRCA-deficient cells and used a lentiviral construct and CRISPR/Cas9-mediated genome editing from VectorBuilder to create our knockout cell lines. The VectorBuilder support team have great technical expertise, are very supportive and fantastic to work with. I highly recommend VectorBuilder's services to accelerate research projects.
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