Codon Optimization

Codons play a critical role in protein synthesis by constituting the genetic code that governs the process of translating information contained in a DNA or RNA sequence into the corresponding protein sequence.

The same amino acid may be encoded by multiple codons and different organisms exhibit preferential usage of certain codons over the others. Although the reasons underlying the existence of such codon usage bias is not known, it does have a significant impact on protein expression. Therefore, it is critical to optimize a gene sequence based on an organism’s codon bias to achieve optimal expression levels in recombinant protein expression studies. This is particularly important in heterologous protein expression systems where the gene of interest (GOI) derived from a particular organism is expressed in a host organism that does not naturally express that gene.

VectorBuilder’s Codon Optimization tool is designed to help you achieve the optimal codon adaptation index (CAI) for your GOIs in any organism of your choice. It includes a comprehensive list of species and is seamlessly incorporated into our online vector design platform enabling you to optimize your GOIs while designing vectors. Additionally, it allows you to avoid cleavage sites of selected restriction enzymes while codon optimizing your target sequence. Our tool can be used for optimizing sequences with extreme GC content and simple repeats for highly efficient gene synthesis and DNA cloning applications.

Note: Sequences in both GenBank and FASTA formats can be recognized by this tool.