VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.
Novartis (Basel), Switzerland
VectorBuilder offers a comprehensive online platform for vector design. We found the options for customising our vectors to be extensive and the interface simple - allowing this to be done ‘with a click’. The team has been really responsive when I wanted to check things through - thanks Christina! In my view, VectorBuilder is a platform worth investigating when you are looking to save time and outsource cloning and virus production.
We find that it is more efficacious to use VectorBuilder for our molecular biology needs. Purchasing vectors and making use of cloning services frees our students’ time to concentrate on experiments. We have worked with VectorBuilder for quite some time and are always pleased with the efficient, effective, and affordable service. In particular, we are impressed by, and appreciate, how well our rep., Justin Mirus, takes care of our needs. VectorBuilder contributes money and expertise to the advancement of science, for example, to enable conferences at our institute.
Jared Sterneckert's lab
Center for Regenerative Therapies, Germany
We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.
Research institute for Rare Diseases, Spain
I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this
point,until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with
cloning,and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.
I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Vancouver Prostate Centre, Canada
Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.
David M Brown
University of Nottingham, UK
We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.
The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.
After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the
plasmidswe provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.
We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.
instituteof Technology, Germany
VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.
TC Biopharm, UK
I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual
gRNAvector, I was able to successfully generate KO cell lines!
I recommend VectorBuilder to CRISPRCas9 users without any hesitation.
Anna Salamero Boix
Georg-Speyer-Haus Frankfurt, Germany
I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.
The First Affiliated Hospital, Sun Yat-sen University, China
The services provided by VectorBuilder are excellent. I had a few vector constructs made by the VectorBuilder. The description of the constructs by their software is excellent. The technical team of the company seems to have a sound knowledge and the technical suggestion regarding vector construction given to me by them were excellent.
Quaild-i-Azame University, Pakistan
I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was also satisfactory.
Southern Medical University, China
I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.
H. Lee Moffitt Cancer Center & Research Institute, USA
Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable prices.
The First Affiliated Hospital of Guangzhou Medical University, China
I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received
quickresponses from the customer support team.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China
I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.
Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK
The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without
Max Planck Institute for Heart and Lung Research, Germany
VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staff were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.
School of Nature Conservation, Beijing Forestry University, China
I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!
University of California San Diego, USA
I must say, having dealt with many vector suppliers up until now, VectorBuilder has been by far the best to communicate rapidly, listening to client requests and going above and beyond wet lab work. As a relatively new company - Their Future is bright.
Vectorbuilder offers a unique platform for easy design of customized DNA vectors and is one of our first choice providers for molecular biology services. Consistently fast turnover times, high quality of services and excellent customer liaison are the decisive points for us to rely on Vectorbuilder's expertise. I can highly recommend Vectorbuilder as a reliable service provider and thank the whole team for the commitment to meeting our needs.
Evotec SE, Germany
VectorBuilder provides fast and economic service for plasmids construction, which saves a lot of time for me. I mostly construct lentivirus plasmids with them and so far have a positive impression for their products. They respond to my requests quickly and honestly so the communication with VectorBuilder’s staffs is one of the best parts of the service.
Lundquist Institute at Harbor-UCLA Medical Center, USA
I’ve been using VectorBuilder’s virus packaging services for two years. Their viruses worked well and my projects are going on well. I’ve published two papers on SCI journals using VectorBuilder’s services. Thank you, VectorBuilder!
Zhejiang University, China
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Dailing et al.
ACS Appl Mater Interfaces. PMID: 33124813 (2020).
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