Promoter: The promoter that drives your gene of interest is placed here. When the inducible GAL1 promoter is used, galactose will induce high-level transcription of the gene of interest, while glucose will strongly repressed expression.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
CYC1 terminator: Sequence which facilitates transcriptional termination and polyadenylation of mRNA in yeast.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
Marker: A yeast selectable marker is placed here. It allows the yeast cells successfully transformed with the vector to be selected. One commonly used marker is the orotidine-5'-phosphate decarboxylase (URA3) gene, which allows selection of yeast transformants in uracil or uridine deficient medium. Additionally, if 5-Fluoroorotic acid (5-FOA) is added to the media, the URA3 gene product will convert 5-FOA into 5-fluorouracil, which is a toxin that will cause cell death, thereby allowing selection against yeast carrying the plasmid.
2µ ori: Origin of replication which permits high-copy replication and maintenance in S. cerevisiae.