CMV promoter: Human cytomegalovirus immediate early promoter. It can drive high-level transcription of the downstream viral antigenomic RNA in packaging cells.
T7 promoter: Promoter from T7 bacteriophage. The T7 RNA polymerase from the phage recognizes this promoter to drive high-level transcription of the downstream viral antigenomic RNA.
VSV-N: Nucleoprotein gene of vesicular stomatitis virus. Assembles into an intact RNase-resistant nucleocapsid as N/RNA polymer; required for initiating viral genome synthesis.
VSV-P: Phosphoprotein gene of vesicular stomatitis virus. Functions as a critical component of the VSV polymerase complex by positioning the large (L) protein on the N/RNA template and stimulating viral RNA synthesis with the L protein at both the initiation and elongation steps.
VSV-M: Matrix protein gene of vesicular stomatitis virus. Associates with the N/RNA nucleocapsid and facilitates virus assembly by promoting condensation of the ribonucleocapsid.
Kozak: Kozak translation initiation sequence. Facilitates translation initiation of ATG start codon downstream of the Kozak sequence.
ORF: The open reading frame of your gene of interest is placed here.
Marker: A drug selection gene (such as neomycin resistance), a visually detectable gene (such as EGFP), or a dual-reporter gene (such as EGFP/Neo). This allows cells transduced with the vector to be selected and/or visualized.
VSV-L: Large polymerase protein gene of vesicular stomatitis virus. Forms RNA-dependent RNA polymerase (RdRp) complexes with the VSV phosphoprotein and catalyzes synthesis of the N, P, M, G, L mRNAs sequentially.
HDV: Hepatitis delta virus (HDV) antigenome self-cleaving ribozyme. This ribozyme, when present in the transcript, catalyzes the self-cleavage of the transcript in cis.
T7 terminator: Transcriptional termination signal from T7 bacteriophage. Allows transcription termination of RNA transcribed by bacteriophage T7 RNA polymerase.
BGH pA: Bovine growth hormone polyadenylation signal. Transcription driven by the CMV promoter is terminated within this sequence.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.