Premade Control Virus
VectorBuilder는 렌티바이러스, AAV, 아데노바이러스, MMLV, MSCV 및 VSV를 위한 인기 바이러스 벡터 컨스트럭트를 포함하여 다양한 바이러스 시스템과 응용 분야에 걸쳐 실험에 적합한 control 바이러스를 제공합니다. 다양한 스케일로 제공되는 이러한 바이러스는 in vitro 및 in vivo 실험을 모두 커버하므로 실험에 적합한 control을 선택할 수 있습니다.
중점 사항
고품질, 고역가 바이러스
- 실험적으로 검증
- 역가 및 무균성 측정을 포함한 엄격한 QC
몇 번의 클릭만으로 주문
- 다양한 스케일 주문 가능
- 선택 가능한 다양한 형광 및 항생제 마커
전문가가 제작
- 벡터 디자인, 클로닝 및 패키징 분야에서 수십 년의 경험
- 최적화된 벡터 및 패키징 프로세스
주문 정보
| Vector System | Vector Name | Scale | Catalog | Price (USD) | Buy Now |
|---|---|---|---|---|---|
| VSV gene expression | pVSV[Exp]-EGFP |
Medium 107 PFU/ml (10x100 ul) |
VSVM(VB010000-9315gcp) | $549 | Please inquire |
|
Large 108 PFU/ml (10x100 ul) |
VSVL(VB010000-9315gcp) | $699 | Please inquire | ||
|
Ultra-purified medium 108 PFU/ml (5x100 ul) |
VSVMP(VB010000-9315gcp) | $849 | Please inquire | ||
|
Ultra-purified large 108 PFU/ml (10x100 ul) |
VSVLP(VB010000-9315gcp) | $1,349 | Please inquire |
정보 자료
주요 인용 논문
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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FAQ
For in vitro, we recommend starting with smaller scales (mini or pilot) to initially test the virus in your experimental system, prior to scaling up to medium/large scales for in vitro experiments and ultra-purified scales for both in vitro and in vivo experiments. Please refer to the table below for details on how the virus has been purified for each of our viral systems.
| Scale | Application | Lentivirus | AAV | Adenovirus | MMLV | MSCV | VSV |
|---|---|---|---|---|---|---|---|
| Mini | Cell culture | PEG concentrated | PEG concentrated | Centrifugation | PEG concentrated | PEG concentrated | PEG concentrated |
| Pilot | |||||||
| Medium | |||||||
| Large | Ultrafritration | ||||||
| Ultra-purified medium | Cell culture and in vivo | Sucrose gradient | CsCl gradient | CsCl gradient | Sucrose gradient | Sucrose gradient | Sucrose gradient |
| Ultra-purified large |