Premade Control Virus
VectorBuilder는 lentivirus, AAV, adenovirus, MMLV, MSCV 및 VSV를 위한 인기 바이러스 벡터 컨스트럭트를 포함하여 다양한 바이러스 시스템과 응용 분야에 걸쳐 실험에 적합한 control 바이러스를 제공합니다. 다양한 스케일로 제공되는 이러한 바이러스는 in vitro 및 in vivo 실험을 모두 커버하므로 실험에 적합한 control을 선택할 수 있습니다.
중점 사항
고품질, 고역가 바이러스
- 실험적으로 검증
- 역가 및 무균성 측정을 포함한 엄격한 QC
몇 번의 클릭만으로 주문 가능
- 다양한 스케일로 주문 가능
- 선택 가능한 다양한 형광 및 항생제 마커
전문가에 의한 제작<
- 벡터 디자인, 클로닝 및 패키징 분야에서 수십 년의 경험
- 최적화된 벡터 및 패키징 프로세스
주문 정보
Lentivirus
AAV
Adenovirus
MMLV
MSCV
VSV
| Vector System | Vector Name | Scale | Catalog | Price (USD) | Buy Now |
|---|---|---|---|---|---|
| VSV gene expression | pVSV[Exp]-EGFP |
Medium
107 PFU/ml (10x100 ul)
|
VSVM(VB010000-9315gcp) | $549 | Please inquire |
|
Large
108 PFU/ml (10x100 ul)
|
VSVL(VB010000-9315gcp) | $699 | |||
|
Ultra-purified medium
108 PFU/ml (5x100 ul)
|
VSVMP(VB010000-9315gcp) | $849 | |||
|
Ultra-purified large
108 PFU/ml (10x100 ul)
|
VSVLP(VB010000-9315gcp) | $1,349 |
정보 자료
주요 인용 논문
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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FAQ
바이러스를 처음 테스트하는 경우에는 작은 스케일 (mini 또는 pilot)로 시작한 다음, in vitro 실험은 medium/large 스케일로 확장하거나, in vivo (및 in vitro) 실험의 경우는 ultra-purified 스케일로 확장하는 것이 좋습니다. 각 바이러스 시스템에서 바이러스가 어떻게 정제되었는지에 대한 자세한 내용은 아래 표를 참조하십시오.
| Scale | Application | Lentivirus | AAV | Adenovirus | MMLV | MSCV |
|---|---|---|---|---|---|---|
| Mini | Suitable for most cell culture experiments | PEG concentration | ||||
| Pilot | PEG concentration | Unpurified | PEG concentration | PEG concentration | ||
| Medium | ||||||
| Large | Ultrafiltration | |||||
| Ultra-purified medium | Cell culture and in vivo | Sucrose gradient purification | CsCl gradient | CsCl gradient | Sucrose gradient purification | Sucrose gradient purification |
| Ultra-purified large |
* For pilot studies and easy-to-transfect cells, non-ultra-purified should be suitable; however, for difficult-to-transfect cells like neurons, ultra-purified may be more suitable. Contact us for more information.