Premade Transposon System Vectors
VectorBuilder에서는 piggyBac, Sleeping Beauty 및 Tol2 시스템에 사용할 수 있는 transposon 기반 실험을 위한 고품질 premade 벡터를 제공합니다. Premade transposon 및 transposase 발현 벡터를 페어링하여 숙주 게놈에 영구적으로 integration 할 수 있는 효율적인 non-viral 솔루션을 제공합니다.
주요 특징
즉시 배송 가능한 premade transposon 시스템 벡터 (piggyBac, Sleeping Beauty, and Tol2).
100% 서열이 보장되고, 기능적으로 검증된 벡터 백본과 구성요소.
몇 번의 클릭만으로 plasmid DNA 정제를 포함한 다운스트림 서비스 이용 가능.PiggyBac
VectorBuilder는 과발현, RNAi, CRISPR를 포함한 다양한 응용을 위한 piggyBac control 벡터를 제공합니다. 이러한 벡터는 고효율 유전자 전달을 위해 piggyBac transposase 발현 벡터와 함께 작동하도록 디자인되었습니다.
Gene expression controls
RNAi controls
CRISPR controls
Transposase
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>EGFP | $189 | View and Order |
| pPB[Exp]-EF1A>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>EGFP | $189 | View and Order |
| pPB[Exp]-CMV>EGFP | $189 | View and Order |
| pPB[Exp]-Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pPB[Exp]-CAG>EGFP | $189 | View and Order |
| pPB[TetOn]-TRE>EGFP-rev(CAG>tTS/rtTA) | $189 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[shRNA]-Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-Bsd-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP/Bsd-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>PBase | $189 | View and Order |
| pRP[Exp]-CAG>PBase | $189 | View and Order |
| pRP[Exp]-EF1A>PBase | $189 | View and Order |
Sleeping Beauty
VectorBuilder는 유전자 발현을 위한 control로 premade Sleeping Beauty 벡터를 제공하며, 이는 고효율 유전자 전달을 위해 Sleeping Beauty transposase 발현 벡터와 함께 작동하도록 디자인되었습니다. VectorBuilder의 celDNA donor를 HiExpress™ Sleeping Beauty Transposase IVT mRNA와 함께 사용하면 더욱 뛰어난 효율을 얻을 수 있습니다.
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| celDNA-CMV>EGFP-T2A-Puro | $449 | View and Order |
| pSB[Exp]-CMV>EGFP | $189 | View and Order |
| pSB[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pSB[Exp]-Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pSB[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pSB[Exp]-CMV>EGFP/Puro | $189 | View and Order |
| pSB[Exp]-CAG>EGFP | $189 | View and Order |
| pSB[Exp]-EF1A>ORF_Stuffer | $189 | View and Order |
| pSB[Exp]-CMV>EGFP | $189 | View and Order |
| pSB[Exp]-CAG>EGFP | $189 | View and Order |
Tol2
VectorBuilder는 유전자 발현을 위한 control로 premade Tol2 벡터를 제공하며, 이는 고효율 유전자 전달을 위해 Tol2 transposase 발현 벡터와 함께 작동하도록 디자인되었습니다.
Gene expression controls
Transposase
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pTol2[Exp]-CMV>EGFP | $189 | View and Order |
| pTol2[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pTol2[Exp]-Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pTol2[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pTol2[Exp]-CAG>EGFP | $189 | View and Order |
| pTol2[Exp]-CAG>ORF_Stuffer | $189 | View and Order |
| pTol2[Exp]-Puro-CAG>ORF_Stuffer | $189 | View and Order |
| pTol2[Exp]-Puro-CAG>EGFP | $189 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CAG>Tol2 | $189 | View and Order |
더 많은 옵션을 보려면 아래에서 인기 벡터를 검색하세요.
Search for RefSeq genes (e.g. RHO)
정보 자료
Featured Citations
Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
Yamada, et al.
Cell Press STAR Protocols, 2021, doi: 10.1016/j.xpro.2021.100811
Products used:
- PiggyBac Vector Cloning
Abstract: We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
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Targeted delivery of a PD-1-blocking scFv by CD133-specific CAR-T cells using nonviral Sleeping Beauty transposition shows enhanced antitumour efficacy for advanced hepatocellular carcinoma
Methods: CD133-specific CAR-T cells secreting PD-1 blocking scFv (CD133 CAR-T and PD-1 s cells) were constructed using a sleeping beauty transposon system from minicircle technology, and the antitumour efficacy of CD133 CAR-T and PD-1 s cells was analysed in vitro and in vivo.
Results: A univariate analysis showed that CD133 expression in male patients at the late stage (II and III) was significantly associated with worse progression-free survival (PFS) (P = 0.0057) and overall survival (OS) (P = 0.015), and a multivariate analysis showed a trend toward worse OS (P = 0.041). Male patients with advanced HCC exhibited an approximately 20-fold higher PD-L1 combined positive score (CPS) compared with those with HCC at an early stage. We successfully generated CD133 CAR-T and PD-1 s cells that could secrete PD-1 blocking scFv based on a sleeping beauty system involving minicircle vectors. CD133 CAR-T and PD-1 s cells exhibited significant antitumour activity against HCC in vitro and in xenograft mouse models. Thus, CD133 CAR-T and PD-1 s cells may be a therapeutically tractable strategy for targeting CD133-positive CSCs in male patients with advanced HCC.
Conclusions: Our study provides a nonviral strategy for constructing CAR-T cells that could also secrete checkpoint blockade inhibitors based on a Sleeping Beauty system from minicircle vectors and revealed a potential benefit of this strategy for male patients with advanced HCC and high CD133 expression (median immunohistochemistry score > 2.284).
Keywords: CAR-T; CD133; Hepatocellular carcinoma; Minicircle; PD-1; Sleeping Beauty system.
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Yang, et al.
BMC Medicine, 2023, doi: 10.1186/s12916-023-03016-0
Products used:
- Sleeping Beauty Vector Cloning
Abstract:
Background: CD133 is considered a marker for cancer stem cells (CSCs) in several types of tumours, including hepatocellular carcinoma (HCC). Chimeric antigen receptor-specific T (CAR-T) cells targeting CD133-positive CSCs have emerged as a tool for the clinical treatment of HCC, but immunogenicity, the high cost of clinical-grade recombinant viral vectors and potential insertional mutagenesis limit their clinical application.
Methods: CD133-specific CAR-T cells secreting PD-1 blocking scFv (CD133 CAR-T and PD-1 s cells) were constructed using a sleeping beauty transposon system from minicircle technology, and the antitumour efficacy of CD133 CAR-T and PD-1 s cells was analysed in vitro and in vivo.
Results: A univariate analysis showed that CD133 expression in male patients at the late stage (II and III) was significantly associated with worse progression-free survival (PFS) (P = 0.0057) and overall survival (OS) (P = 0.015), and a multivariate analysis showed a trend toward worse OS (P = 0.041). Male patients with advanced HCC exhibited an approximately 20-fold higher PD-L1 combined positive score (CPS) compared with those with HCC at an early stage. We successfully generated CD133 CAR-T and PD-1 s cells that could secrete PD-1 blocking scFv based on a sleeping beauty system involving minicircle vectors. CD133 CAR-T and PD-1 s cells exhibited significant antitumour activity against HCC in vitro and in xenograft mouse models. Thus, CD133 CAR-T and PD-1 s cells may be a therapeutically tractable strategy for targeting CD133-positive CSCs in male patients with advanced HCC.
Conclusions: Our study provides a nonviral strategy for constructing CAR-T cells that could also secrete checkpoint blockade inhibitors based on a Sleeping Beauty system from minicircle vectors and revealed a potential benefit of this strategy for male patients with advanced HCC and high CD133 expression (median immunohistochemistry score > 2.284).
Keywords: CAR-T; CD133; Hepatocellular carcinoma; Minicircle; PD-1; Sleeping Beauty system.
Serotonergic modulation of vigilance states in zebrafish and mice
Zhao, et al.
Nature Communications, 2024, doi: 10.1038/s41467-024-47021-0
Products used:
Abstract: Vigilance refers to being alertly watchful or paying sustained attention to avoid potential threats. Animals in vigilance states reduce locomotion and have an enhanced sensitivity to aversive stimuli so as to react quickly to dangers.Here we report that an unconventional 5-HT driven mechanism operating at neural circuit level which shapes the internal state underlying vigilance behavior in zebrafish and male mice. The neural signature of internal vigilance state was characterized by persistent low-frequency high-amplitude neuronal synchrony in zebrafish dorsal pallium and mice prefrontal cortex. The neuronal synchronization underlying vigilance was dependent on intense release of 5-HT induced by persistent activation of either DRN 5-HT neuron or local 5-HT axon terminals in related brain regions via activation of 5-HTR7. Thus, we identify a mechanism of vigilance behavior across species that illustrates the interplay between neuromodulators and neural circuits necessary to shape behavior states.
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