Premade Tet System Vectors
VectorBuilder는 leaky 발현을 최소화하도록 설계된 Tet regulatory 단백질을 인코딩하는 다양한 premade 벡터를 제공하여 induction 후 관심 유전자의 강력하고 빠른 발현 (Tet-On) 또는 억제 (Tet-Off)가 가능합니다. 또한 Tet-inducible control vector와 Cas9를 제공하여 유도 유전자 발현 실험을 더욱 간소화하고 연구 효율성을 향상시킵니다.
중점 사항
Viral 및 non-viral 백본으로 제공하는 즉시 배송 가능한 premade Tet 시스템 벡터.
100% 서열이 보장되고, 기능적으로 검증된 벡터 백본과 구성요소.
몇 번의 클릭만으로 plasmid DNA 정제 및 바이러스 패키징을 포함한 다운스트림 서비스 이용 가능.Tet-On Regulators (tTS/rtTA)
VectorBuilder는 Tet-On 시스템에서 사용할 Tet regulatory 단백질의 효율적인 발현을 위한 premade 벡터를 제공하며, 이는 viral 및 non-viral 벡터 백본으로 사용 가능합니다.
 Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-Puro-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Bsd-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Neo-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
 Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Puro-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Hygro-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Bsd-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTS/rtTA | $189 | View and Order |
 AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>tTS/rtTA | $189 | View and Order |
| pAAV[Exp]-CAG>tTS/rtTA:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>tTS/rtTA | $189 | View and Order |
| pAAV[Exp]-CMV>tTS/rtTA:WPRE | $189 | View and Order |
 Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Puro-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Hygro-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Bsd-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Neo-CAG>tTS/rtTA | $189 | View and Order |
Tet-Off Regulator (tTA2)
VectorBuilder는 Tet-Off 시스템에서 사용할 Tet regulatory 단백질의 효율적인 발현을 위한 premade 벡터를 제공하며, 이는 viral 및 non-viral 벡터 백본으로 사용 가능합니다.
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Neo-EF1A>tTA2 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>tTA2 | $189 | View and Order |
| pAAV[Exp]-CAG>tTA2:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>tTA2 | $189 | View and Order |
| pAAV[Exp]-CMV>tTA2:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Neo-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
Tet-Inducible Controls
Tet 조절 유전자 발현에 대한 control로, VectorBuilder는 TRE promoter의 제어 하에 GFP 또는 mCherry를 인코딩하는 premade 벡터를 제공합니다.
Tet-inducible GFP
Tet-inducible mCherry
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-TRE>EGFP | $189 | View and Order |
| pRP[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pRP[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pRP[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Puro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Neo-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-TRE>EGFP-rev(CMV>tTS/rtTA) | $239 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Hygro-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>EGFP | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>EGFP | $189 | View and Order |
| pAAV[Exp]-TRE>EGFP:WPRE | $189 | View and Order |
| pAAV[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>EGFP | $189 | View and Order |
| pPB[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Puro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Hygro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Neo-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Bsd-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-TRE>mCherry | $189 | View and Order |
| pRP[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pRP[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| pRP[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Neo-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Puro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-TRE>mCherry | $189 | View and Order |
| pLV[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pLV[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>mCherry | $189 | View and Order |
| pAAV[Exp]-TRE>mCherry:WPRE | $189 | View and Order |
| pAAV[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>mCherry | $189 | View and Order |
| pPB[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Puro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Neo-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Hygro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Bsd-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
Tet-Inducible Cas9
CRISPR 실험을 엄격하게 제어하기 위해 Tet-inducible Cas9를 인코딩하는 벡터를 제공합니다.
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>SaCas9 | $189 | View and Order |
| pAAV[Exp]-TRE>SaCas9:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-mCherry-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
더 많은 옵션을 보려면 아래에서 인기 벡터를 검색하세요.
Search for RefSeq genes (e.g. RHO)
정보 자료
주요 인용 논문
H3K36 methylation maintains cell identity by regulating opposing lineage programmes
Hoetker, et al.
Nature Cell Biology, 2023, doi: 10.1038/s41556-023-01191-z
Products used:
- Vector Cloning (Mammalian Tet Inducible Gene Expression Lentiviral Vector)
- Vector Cloning (Mammalian shRNA Knockdown Lentiviral Vector)
- shRNA Gene Knockdown Solutions
- Lentivirus Packaging
Abstract: The epigenetic mechanisms that maintain differentiated cell states remain incompletely understood. Here, we employed histone mutants to uncover a crucial role for H3K36-methylation in the maintenance of cell identities across diverse developmental contexts. Focusing on the experimental induction of pluripotency, we show that H3K36M-mediated depletion of H3K36-methylation endows fibroblasts with a plastic state poised to acquire pluripotency in nearly all cells. At a cellular level, H3K36M facilitates epithelial plasticity by rendering fibroblasts insensitive to TGFb signals. At a molecular level, H3K36M enables the decommissioning of mesenchymal enhancers and the parallel activation of epithelial/stem cell enhancers. This enhancer rewiring is Tet-dependent and redirects Sox2 from promiscuous somatic to pluripotency targets. Our findings reveal a previously unappreciated dual role for H3K36-methylation in the maintenance of cell identity by integrating a crucial developmental pathway into sustained expression of cell type-specific programs, and by opposing the expression of alternative lineage programs through enhancer methylation.
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Concurrent inhibition of oncogenic and wild-type RAS-GTP for cancer therapy
Holderfield, et al.
Nature, 2024, doi: 10.1038/s41586-024-07205-6
Products used:
- Vector Cloning (Mammalian Tet Inducible Gene Expression Lentiviral Vector)
- Vector Cloning (Tet Regulatory Protein Expression Lentiviral Vector))
- Lentivirus Packaging
Abstract: RAS oncogenes (collectively NRAS, HRAS and especially KRAS) are among the most frequently mutated genes in cancer, with common driver mutations occurring at codons 12, 13 and 61. Small molecule inhibitors of the KRAS(G12C) oncoprotein have demonstrated clinical efficacy in patients with multiple cancer types and have led to regulatory approvals for the treatment of non-small cell lung cancer. Nevertheless, KRASG12C mutations account for only around 15% of KRAS-mutated cancers, and there are no approved KRAS inhibitors for the majority of patients with tumours containing other common KRAS mutations. Here we describe RMC-7977, a reversible, tri-complex RAS inhibitor with broad-spectrum activity for the active state of both mutant and wild-type KRAS, NRAS and HRAS variants (a RAS(ON) multi-selective inhibitor). Preclinically, RMC-7977 demonstrated potent activity against RAS-addicted tumours carrying various RAS genotypes, particularly against cancer models with KRAS codon 12 mutations (KRASG12X). Treatment with RMC-7977 led to tumour regression and was well tolerated in diverse RAS-addicted preclinical cancer models. Additionally, RMC-7977 inhibited the growth of KRASG12X cancer models that are resistant to KRAS(G12C) inhibitors owing to restoration of RAS pathway signalling. Thus, RAS(ON) multi-selective inhibitors can target multiple oncogenic and wild-type RAS isoforms and have the potential to treat a wide range of RAS-addicted cancers with high unmet clinical need. A related RAS(ON) multi-selective inhibitor, RMC-6236, is currently under clinical evaluation in patients with KRAS-mutant solid tumours (ClinicalTrials.gov identifier: NCT05379985).
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학습 자료
벡터 가이드 WebpagesLearn more about the different Tet systems available at VectorBuilder by clicking here: Inducible gene expression solutions.