Premade Control Vectors
유전자 과발현에서 shRNA knockdown 및 CRISPR 실험까지, VectorBuilder는 연구 요구사항을 충족시키기 위해 다양한 premade control 벡터를 제공합니다. 실험에 바로 사용 가능한 바이러스에 대해서는 Premade Control Viruses 페이지를 방문하세요.
중점 사항
Viral 및 non-viral 백본으로 제공하는 즉시 배송 가능한 premade 벡터 컬렉션.
100% 서열이 보장되고, 기능적으로 검증된 벡터 백본과 구성요소.
몇 번의 클릭만으로 plasmid DNA 정제 및 바이러스 패키징을 포함한 다운스트림 서비스 이용 가능.Fluorescent Reporters
VectorBuilder는 premade 형광 리포터 plasmid의 방대한 컬렉션을 제공하며, 단색 컬러, 멀티 컬러 및 fluorescence energy transfer 실험의 효율성을 향상시킵니다. 더 많은 형광 단백질 및 컨스트럭트 옵션에 대해서는 인기 벡터 검색 기능을 사용하거나 인기 ORFs를 확인할 수 있습니다.
GFP
mCherry
TagBFP2
 Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>EGFP | $189 | View and Order |
| pRP[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pRP[Exp]-mCherry-CMV>EGFP | $189 | View and Order |
| pRP[Exp]-CAG>EGFP | $189 | View and Order |
| pRP[Exp]-Puro-CAG>EGFP | $189 | View and Order |
| pRP[Exp]-mCherry-CAG>EGFP | $189 | View and Order |
| pRP[Exp]-EF1A>EGFP | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>EGFP | $189 | View and Order |
| pRP[Exp]-mCherry-EF1A>EGFP | $189 | View and Order |
 Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>EGFP | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>EGFP | $189 | View and Order |
| pLV[Exp]-mCherry-EF1A>EGFP | $189 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>mCherry | $189 | View and Order |
| pLV[Exp]-CMV>EGFP | $189 | View and Order |
| pLV[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pLV[Exp]-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>EGFP | $189 | View and Order |
| pLV[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
 AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>EGFP | $189 | View and Order |
| pAAV[Exp]-CAG>EGFP:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>EGFP | $189 | View and Order |
| pAAV[Exp]-CMV>EGFP:WPRE | $189 | View and Order |
| pscAAV[Exp]-CMV>EGFP | $189 | View and Order |
| pAAV[Exp]-SYN1>EGFP | $189 | View and Order |
| pscAAV[Exp]-SYN1>EGFP | $189 | View and Order |
| pAAV[Exp]-SYN1>EGFP:WPRE | $189 | View and Order |
 Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>EGFP | $189 | View and Order |
| pAd5/F35[Exp]-CMV>EGFP | $189 | View and Order |
| pAV[Exp]-EF1A>EGFP | $189 | View and Order |
| pAd5/F35[Exp]-EF1A>EGFP | $189 | View and Order |
 Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>EGFP | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>EGFP | $189 | View and Order |
| pPB[Exp]-CAG>EGFP | $189 | View and Order |
| pPB[Exp]-Puro-CAG>EGFP | $189 | View and Order |
| pPB[Exp]-CMV>EGFP | $189 | View and Order |
| pPB[Exp]-Puro-CMV>EGFP | $189 | View and Order |
| pPB[Exp]-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pSB[Exp]-EF1A>EGFP | $189 | View and Order |
| pSB[Exp]-EF1A>EGFP/Puro | $189 | View and Order |
| pSB[Exp]-CMV>EGFP | $189 | View and Order |
| pSB[Exp]-CMV>EGFP/Puro | $189 | View and Order |
| pSB[Exp]-CAG>EGFP | $189 | View and Order |
| pSB[Exp]-CAG>EGFP/Puro | $189 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>mCherry | $189 | View and Order |
| pRP[Exp]-Puro-CMV>mCherry | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>mCherry | $189 | View and Order |
| pRP[Exp]-EF1A>mCherry | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>mCherry | $189 | View and Order |
| pRP[Exp]-EGFP-EF1A>mCherry | $189 | View and Order |
| pRP[Exp]-CAG>mCherry | $189 | View and Order |
| pRP[Exp]-Puro-CAG>mCherry | $189 | View and Order |
| pRP[Exp]-EGFP-CAG>mCherry | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>mCherry | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>mCherry | $189 | View and Order |
| pLV[Exp]-EGFP-EF1A>mCherry | $189 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>mCherry | $189 | View and Order |
| pLV[Exp]-CMV>mCherry | $189 | View and Order |
| pLV[Exp]-Puro-CMV>mCherry | $189 | View and Order |
| pLV[Exp]-TRE>mCherry | $189 | View and Order |
| pLV[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>mCherry | $189 | View and Order |
| pAAV[Exp]-CAG>mCherry:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>mCherry | $189 | View and Order |
| pAAV[Exp]-CMV>mCherry:WPRE | $189 | View and Order |
| pAAV[Exp]-SYN1>mCherry | $189 | View and Order |
| pAAV[Exp]-SYN1>mCherry:WPRE | $189 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>mCherry | $189 | View and Order |
| pAd5/F35[Exp]-CMV>mCherry | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>mCherry | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>mCherry | $189 | View and Order |
| pPB[Exp]-CAG>mCherry | $189 | View and Order |
| pPB[Exp]-Puro-CAG>mCherry | $189 | View and Order |
| pPB[Exp]-CMV>mCherry | $189 | View and Order |
| pPB[Exp]-Puro-CMV>mCherry | $189 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>TagBFP2 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>TagBFP2 | $189 | View and Order |
| pRP[Exp]-EF1A>TagBFP2 | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>TagBFP2 | $189 | View and Order |
| pRP[Exp]-CAG>TagBFP2 | $189 | View and Order |
| pRP[Exp]-Puro-CAG>TagBFP2 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>TagBFP2 | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>TagBFP2 | $189 | View and Order |
| pLV[Exp]-CMV>TagBFP2 | $189 | View and Order |
| pLV[Exp]-Puro-CMV>TagBFP | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>TagBFP2 | $189 | View and Order |
| pAAV[Exp]-CAG>TagBFP2:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>TagBFP2 | $189 | View and Order |
| pAAV[Exp]-CMV>TagBFP2:WPRE | $189 | View and Order |
| pAAV[Exp]-SYN1>TagBFP2 | $189 | View and Order |
| pAAV[Exp]-SYN1>TagBFP2:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>TagBFP2 | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>TagBFP2 | $189 | View and Order |
| pPB[Exp]-CAG>TagBFP2 | $189 | View and Order |
| pPB[Exp]-Puro-CAG>TagBFP2 | $189 | View and Order |
| pPB[Exp]-CMV>TagBFP2 | $189 | View and Order |
| pPB[Exp]-Puro-CMV>TagBFP2 | $189 | View and Order |
Control shRNA
Control shRNA Vectors
VectorBuilder는 많은 생물학 응용 분야에서 control 벡터로 사용하기에 적합한 scramble shRNA 또는 특정한 유전자를 타겟으로 하는 shRNA를 발현하는 shRNA 벡터 패널을 제공합니다. Plasmid DNA 정제 및 바이러스 패키징은 이러한 벡터를 shopping cart에 추가할 때 downstream 서비스로 구입할 수 있습니다.
아래의 벡터 picker를 사용하여 U6 기반의 shRNA 벡터를 주문할 수 있습니다.
miR30 기반의 shRNA 벡터를 주문하려면, 원하시는 사항을 디자인 의뢰하기로 보내주시면 됩니다..
Scramble shRNA Vectors
RNAi 실험에 대한 음성 대조군으로서, VectorBuilder는 인간이나 마우스 유전체의 어떤 서열도 인식하지 않는 shRNA를 인코딩하는 scramble shRNA control plasmid를 제공합니다.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[shRNA]-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-Puro-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-Hygro-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pRP[shRNA]-mCherry/Neo-U6>Scramble_shRNA | $149 | View and Order |
| pRP[miR30]-EGFP/Puro-EF1A>mCherry:Scramble_miR30-shRNA | $299 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[shRNA]-Puro-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-Neo-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-Hygro-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-EGFP/Neo-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-LacI/Puro-U6/2xLacO>Scramble_shRNA | $149 | View and Order |
| pLV[shRNA]-TetR:IRES:Puro-U6/2xTetO>Scramble_shRNA | $149 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[shRNA]-CAG>EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pAAV[shRNA]-CAG>mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pscAAV[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pscAAV[shRNA]-mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pAAV[miR30]-CAG>EGFP:Scramble_miR30-shRNA:WPRE | $299 | View and Order |
| pAAV[miR30]-CAG>mCherry:Scramble_miR30-shRNA:WPRE | $299 | View and Order |
| pAAV[miR30]-CMV>EGFP:Scramble_miR30-shRNA:WPRE | $299 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[shRNA]-EGFP-U6>Scramble_shRNA | $299 | View and Order |
| pAV[shRNA]-mCherry-U6>Scramble_shRNA | $299 | View and Order |
| pAV[shRNA]-TagBFP2-U6>Scramble_shRNA | $299 | View and Order |
| pAV[miR30]-CMV>EGFP:Scramble_miR30-shRNA | $299 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[shRNA]-Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-Bsd-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-EGFP/Bsd-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149 | View and Order |
| pPB[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149 | View and Order |
Non-Targeting gRNA
CRISPR 실험의 음성 대조군으로서, VectorBuilder는 gRNA를 인코딩하는 non-targeting/scramble gRNA plasmid와 gRNA 및 Cas9를 인코딩하는 이중 발현 벡터를 제공합니다. Control gRNA는 인간 또는 마우스 게놈의 어떤 서열도 인식하지 못합니다.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pRP[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[2gRNA]-EGFP-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2CRISPR]-EGFP-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[gRNA]-Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[2gRNA]-EGFP-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[CRISPR]-hCas9/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[2CRISPR]-hCas9/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[gRNA]-mCherry-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[msgRNA]-Puro-U6>Scramble_msgRNA | $149 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[SagRNA]-CAG>EGFP-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[SagRNA]-CAG>EGFP/Puro-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[CRISPR]-SaCas9-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[msSagRNA]-MS2/P65/HSF1-U6>Scramble_msSagRNA | $189 | View and Order |
| pAAV[gRNA]-CAG>EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pAAV[gRNA]-CAG>EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[gRNA]-EGFP-U6>Scramble_gRNA | $299 | View and Order |
| pAV[CRISPR]-hCas9-U6>Scramble_gRNA | $299 | View and Order |
| pAV[CRISPR]-hCas9/EGFP-U6>Scramble_gRNA | $299 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
Negative Controls
유전자 과발현 실험을 위한 병행 음성 대조군으로서, VectorBuilder는 E. coli beta-galactosidase의 아미노산 2~83을 인코딩하는 ORF stuffer 발현 벡터를 제공합니다.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-EGFP/Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-mCherry-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-mCherry/Puro-CMV>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-CAG>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-EGFP-CAG>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-EGFP/Puro-CAG>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-mCherry-CAG>ORF_Stuffer | $189 | View and Order |
| pRP[Exp]-mCherry/Puro-CAG>ORF_Stuffer | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-EGFP-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-mCherry-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-mCherry/Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-mCherry/Neo-EF1A>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pLV[Exp]-Puro-CMV>ORF_Stuffer | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>ORF_Stuffer | $189 | View and Order |
| pAAV[Exp]-CAG>ORF_Stuffer:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pAAV[Exp]-CMV>ORF_Stuffer:WPRE | $189 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>ORF_Stuffer | $189 | View and Order |
| pAV[Exp]-EGFP-CMV>ORF_Stuffer | $189 | View and Order |
| pAV[Exp]-mCherry-CMV>ORF_Stuffer | $189 | View and Order |
| pAV[Exp]-EF1A>ORF_Stuffer | $189 | View and Order |
| pAV[Exp]-EGFP-EF1A>ORF_Stuffer | $189 | View and Order |
| pAV[Exp]-mCherry-EF1A>ORF_Stuffer | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-CAG>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-Puro-CAG>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-CAG>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-mCherry/Puro-CAG>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-EF1A>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-EF1A>ORF_Stuffer | $189 | View and Order |
| pPB[Exp]-mCherry/Puro-EF1A>ORF_Stuffer | $189 | View and Order |
더 많은 옵션을 보려면 아래에서 인기 벡터를 검색하세요.
Search for RefSeq genes (e.g. RHO)
정보 자료
주요 인용 논문
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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